lhr74 antibodies Search Results


lhr74 antibodies  (ATCC)
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ATCC lhr74 antibodies
Epitope mapping of LHR29 monoclonal antibody. a ) Three recombinant proteins containing 229, 291 or 318 amino acid residues of the LHCGR extracellular domain and C-terminal FLAG-epitope were expressed in CHO cells as described ; b ), recombinant as well as mock transfected extracts (no DNA) were resolved in SDS-PAGE, blotted and probed with anti-FLAG, LHR29 and PG732 mono- and polyclonal antibodies respectively; c ), two monoclonal antibodies <t>(LHR74</t> and LHR29) and one polyclonal (PG732) were used in western blot analysis of proteins extracted from placental extracts at 12 wks of gestation.
Lhr74 Antibodies, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Epitope mapping of LHR29 monoclonal antibody. a ) Three recombinant proteins containing 229, 291 or 318 amino acid residues of the LHCGR extracellular domain and C-terminal FLAG-epitope were expressed in CHO cells as described ; b ), recombinant as well as mock transfected extracts (no DNA) were resolved in SDS-PAGE, blotted and probed with anti-FLAG, LHR29 and PG732 mono- and polyclonal antibodies respectively; c ), two monoclonal antibodies (LHR74 and LHR29) and one polyclonal (PG732) were used in western blot analysis of proteins extracted from placental extracts at 12 wks of gestation.

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Quantitative ELISAs for serum soluble LHCGR and hCG-LHCGR complex: potential diagnostics in first trimester pregnancy screening for stillbirth, Down’s syndrome, preterm delivery and preeclampsia

doi: 10.1186/1477-7827-10-113

Figure Lengend Snippet: Epitope mapping of LHR29 monoclonal antibody. a ) Three recombinant proteins containing 229, 291 or 318 amino acid residues of the LHCGR extracellular domain and C-terminal FLAG-epitope were expressed in CHO cells as described ; b ), recombinant as well as mock transfected extracts (no DNA) were resolved in SDS-PAGE, blotted and probed with anti-FLAG, LHR29 and PG732 mono- and polyclonal antibodies respectively; c ), two monoclonal antibodies (LHR74 and LHR29) and one polyclonal (PG732) were used in western blot analysis of proteins extracted from placental extracts at 12 wks of gestation.

Article Snippet: Purified LHR29 and LHR74 antibodies were initially provided by Dr Hugues Loosfelt (INSERM, France) and subsequently the antibody producing clones were obtained from ATCC (Clone ID CRL-2685 and CRL-2686).

Techniques: Recombinant, FLAG-tag, Transfection, SDS Page, Bioprocessing, Western Blot